MYPT1 (Phospho Thr853) Rabbit pAb
-YP0662
主要信息
Target
MYPT1 Phospho Thr853
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
IF, WB, IHC, ELISA
MW
130kD (Observed)
Conjugate/Modification
Phospho
货号: YP0662
规格
价格
货期
数量
200μL
￥4,680.00
现货
0
100μL
￥2,800.00
现货
0
50μL
￥1,500.00
现货
0
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详细信息
推荐稀释比
IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; Not yet tested in other applications;
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
特异性
Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RStGV
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
实测条带
130kD
修饰
Phospho
克隆性
Polyclonal
同种型
IgG
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抗原&靶点信息
免疫原:
The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr853. AA range:621-670
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特异性:
Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RStGV
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基因名称:
PPP1R12A
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蛋白名称:
Protein phosphatase 1 regulatory subunit 12A
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别名:
PPP1R12A ;
MBS ;
MYPT1 ;
Protein phosphatase 1 regulatory subunit 12A ;
Myosin phosphatase-targeting subunit 1 ;
Myosin phosphatase target subunit 1 ;
Protein phosphatase myosin-binding subunit
MBS ;
MYPT1 ;
Protein phosphatase 1 regulatory subunit 12A ;
Myosin phosphatase-targeting subunit 1 ;
Myosin phosphatase target subunit 1 ;
Protein phosphatase myosin-binding subunit
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背景:
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph
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功能:
Function:Regulates myosin phosphatase activity.,PTM:Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 6 ANK repeats.,subcellular location:Along actomyosin filaments and stress fibers.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.,
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细胞定位:
Cytoplasm . Cytoplasm, cytoskeleton, stress fiber . Also along actomyosin filaments. .
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研究领域:
>>cGMP-PKG signaling pathway ;
>>cAMP signaling pathway ;
>>Vascular smooth muscle contraction ;
>>Focal adhesion ;
>>Platelet activation ;
>>Regulation of actin cytoskeleton ;
>>Oxytocin signaling pathway ;
>>Proteoglycans in cancer
>>cAMP signaling pathway ;
>>Vascular smooth muscle contraction ;
>>Focal adhesion ;
>>Platelet activation ;
>>Regulation of actin cytoskeleton ;
>>Oxytocin signaling pathway ;
>>Proteoglycans in cancer
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信号通路
Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion
Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton
Organismal Systems >> Immune system >> Platelet activation
Organismal Systems >> Endocrine system >> Oxytocin signaling pathway
Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction
Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway
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货号: YP0662
规格
价格
货期
数量
200μL
￥4,680.00
现货
0
100μL
￥2,800.00
现货
0
50μL
￥1,500.00
现货
0
加入购物车
已收藏
收藏
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