The antiserum was produced against synthesized peptide derived from human EPB41 around the phosphorylation site of Tyr660/418. AA range:626-675

Phospho-4.1R (Y660) Polyclonal Antibody detects endogenous levels of 4.1R protein only when phosphorylated at Y660.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):NIyIR

EPB41

Protein 4.1

EPB41 ;
E41P ;
Protein 4.1 ;
P4.1 ;
4.1R ;
Band 4.1 ;
EPB4.1

The protein encoded by this gene , together with spectrin and actin , constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1) . Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq , Oct 2009] ,

Disease:Defects in EPB41 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive hematologic disorder characterized by hemolytic anemia , microspherocytosis , poikilocytosis , and an unusual thermal sensitivity of red cells. ,Disease:Defects in EPB41 are the cause of elliptocytosis type 1 (EL1) [MIM:611804]. EL1 is a Rhesus-linked form of hereditary elliptocytosis , a genetically heterogeneous , autosomal dominant , hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. ,Function:Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. ,PTM:O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain. ,PTM:Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions. ,PTM:Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex. ,similarity:Contains 1 FERM domain. ,subunit:Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin , CENPJ and DLG1. Also found to associate with contractile apparatus and tight junctions. ,

Cytoplasm , cytoskeleton . Cytoplasm , cell cortex . Nucleus .