The antiserum was produced against synthesized peptide derived from human PBK/TOPK around the phosphorylation site of Thr9. AA range:1-50

Phospho-PBK (T9) Polyclonal Antibody detects endogenous levels of PBK protein only when phosphorylated at T9.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):FKtPS

PBK

Lymphokine-activated killer T-cell-originated protein kinase

PBK ;
TOPK ;
Lymphokine-activated killer T-cell-originated protein kinase ;
Cancer/testis antigen 84 ;
CT84 ;
MAPKK-like protein kinase ;
Nori-3 ;
PDZ-binding kinase ;
Spermatogenesis-related protein kinase ;
SPK ;
T-LAK cell-originated protein kinas

PDZ binding kinase (PBK) Homo sapiens This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions , with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq , Jul 2013] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,enzyme regulation:Activated by phosphorylation. ,Function:Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated , forms a complex with TP53 , leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. ,PTM:Phosphorylated; in a cell-cycle dependent manner at mitosis. ,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily. ,similarity:Contains 1 protein kinase domain. ,subunit:Interacts with DLG1 and TP53. ,tissue specificity:Expressed in the testis and placenta. In the testis , restrictedly expressed in outer cell layer of seminiferous tubules. ,

nucleus ,