Hck (Phospho Tyr521) Rabbit pAb
-YP0494
主要信息
Target
Hck Phospho Tyr521
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
MW
60kD (Observed)
Conjugate/Modification
Phospho
货号: YP0494
规格
价格
货期
数量
200μL
￥4,680.00
现货
0
100μL
￥2,800.00
现货
0
50μL
￥1,500.00
现货
0
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详细信息
推荐稀释比
WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
特异性
Phospho-Hck (Y521) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y521.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SQyQQ
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
实测条带
60kD
修饰
Phospho
克隆性
Polyclonal
同种型
IgG
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抗原&靶点信息
免疫原:
Synthesized phospho-peptide around the phosphorylation site of human Hck (phospho Tyr521)
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特异性:
Phospho-Hck (Y521) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y521.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SQyQQ
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基因名称:
HCK
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蛋白名称:
Tyrosine-protein kinase HCK
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别名:
HCK ;
Tyrosine-protein kinase HCK ;
Hematopoietic cell kinase ;
Hemopoietic cell kinase ;
p59-HCK/p60-HCK ;
p59Hck ;
p61Hck
Tyrosine-protein kinase HCK ;
Hematopoietic cell kinase ;
Hemopoietic cell kinase ;
p59-HCK/p60-HCK ;
p59Hck ;
p61Hck
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背景:
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010],
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功能:
Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Domain:The SH3 domain mediates binding to HIV-1 Nef.,Function:May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.,PTM:Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:May interact (via SH3 domain) with HIV-1 Nef and Vif. This interaction would stimulates its tyrosine-kinase activity. Interacts (via SH3 domain) with HEV ORF3 protein.,tissue specificity:Expressed predominantly in cells of the myeloid and B-lymphoid lineages.,
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细胞定位:
[Isoform 1]: Lysosome. Membrane; Lipid-anchor. Cell projection, podosome membrane; Lipid-anchor. Cytoplasm, cytosol. Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.; [Isoform 2]: Cell membrane ; Lipid-anchor . Membrane, caveola ; Lipid-anchor . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Golgi apparatus . Cytoplasmic vesicle . Lysosome . Nucleus . 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions.; Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.
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研究领域:
>>Chemokine signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Kaposi sarcoma-associated herpesvirus infection
>>Fc gamma R-mediated phagocytosis ;
>>Kaposi sarcoma-associated herpesvirus infection
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信号通路
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货号: YP0494
规格
价格
货期
数量
200μL
￥4,680.00
现货
0
100μL
￥2,800.00
现货
0
50μL
￥1,500.00
现货
0
加入购物车
已收藏
收藏
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