The antiserum was produced against synthesized peptide derived from human MKK3 around the phosphorylation site of Ser189. AA range:173-222

Phospho-MEK-3 (S189) Polyclonal Antibody detects endogenous levels of MEK-3 protein only when phosphorylated at S189.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):VDsVA

MAP2K3

Dual specificity mitogen-activated protein kinase kinase 3

MAP2K3 ;
MEK3 ;
MKK3 ;
PRKMK3 ;
SKK2 ;
Dual specificity mitogen-activated protein kinase kinase 3 ;
MAP kinase kinase 3 ;
MAPKK 3 ;
MAPK/ERK kinase 3 ;
MEK 3 ;
Stress-activated protein kinase kinase 2 ;
SAPK kinase 2 ;
SAPKK-2 ;
SAPKK2

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress , and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin , and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase , which thus leads to the constitutive activation of MAPK14 , and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq , Jul 2008] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,Disease:Defects in MAP2K3 may be involved in colon cancer. ,enzyme regulation:Activated by dual phosphorylation on Ser-218 and Thr-222. ,Function:Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. ,PTM:Autophosphorylated. ,PTM:Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. ,PTM:Yersinia yopJ may acetylate Ser/Thr residues , preventing phosphorylation and activation , thus blocking the MAPK signaling pathway. ,similarity:Belongs to the protein kinase superfamily. ,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. ,similarity:Contains 1 protein kinase domain. ,subunit:Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3 , RAC1 and CCM2. Interacts with Yersinia yopJ. ,tissue specificity:Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. ,

nucleoplasm ,cytoplasm ,cytosol ,membrane ,

>>MAPK signaling pathway ;
>>Rap1 signaling pathway ;
>>Cellular senescence ;
>>Toll-like receptor signaling pathway ;
>>Fc epsilon RI signaling pathway ;
>>TNF signaling pathway ;
>>Thermogenesis ;
>>Inflammatory mediator regulation of TRP channels ;
>>GnRH signaling pathway ;
>>Growth hormone synthesis , secretion and action ;
>>Alcoholic liver disease ;
>>Amyotrophic lateral sclerosis ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Salmonella infection ;
>>Yersinia infection ;
>>Toxoplasmosis ;
>>Hepatitis B ;
>>Epstein-Barr virus infection ;
>>Human immunodeficiency virus 1 infection ;
>>PD-L1 expression and PD-1 checkpoint pathway in cancer ;
>>Lipid and atherosclerosis