The antiserum was produced against synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser498. AA range:464-513

Phospho-HDAC5 (S498) Polyclonal Antibody detects endogenous levels of HDAC5 protein only when phosphorylated at S498.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):TQsSP

HDAC5

Histone deacetylase 5

HDAC5 ;
KIAA0600 ;
Histone deacetylase 5 ;
HD5 ;
Antigen NY-CO-9

Histones play a critical role in transcriptional regulation , cell cycle progression , and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins , resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jul 2008] ,

Catalytic activity:Hydrolysis of an N (6) -acetyl-lysine residue of a histone to yield a deacetylated histone. ,Domain:The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. ,Function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A , H2B , H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation , cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation , it shuttles into the cytoplasm , allowing the expression of myocyte enhancer factors. ,PTM:Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. ,PTM:Ubiquitinated. Polyubiquitination however does not lead to its degradation. ,similarity:Belongs to the histone deacetylase family. Type 2 subfamily. ,subcellular location:Shuttles between the nucleus and the cytoplasm. In muscle cells , it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. ,subunit:Interacts with AHRR (By similarity) . Interacts with BCOR , HDAC7 , HDAC9 , CTBP1 , MEF2C , NCOR2 , NRIP1 , PHB2 and a 14-3-3 chaperone protein. Interacts with KDM5B. ,tissue specificity:Ubiquitous. ,

Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells , it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK , CaMK1 and SIK1.

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