The antiserum was produced against synthesized peptide derived from human BAD around the phosphorylation site of Ser136. AA range:102-151

Phospho-Bad (S136) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S136.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SRsAP

BAD

Bcl2 antagonist of cell death

BAD ;
BBC6 ;
BCL2L8 ;
Bcl2 antagonist of cell death ;
BAD ;
Bcl-2-binding component 6 ;
Bcl-2-like protein 8 ;
Bcl2-L-8 ;
Bcl-XL/Bcl-2-associated death promoter

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2 , and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase , as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq , Jul 2008] ,

Domain:Intact BH3 motif is required by BIK , BID , BAK , BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. ,Function:Promotes cell death. Successfully competes for the binding to Bcl-X (L) , Bcl-2 and Bcl-W , thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X (L) , but not that of Bcl-2 (By similarity) . Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. ,online information:Bcl 2-associated death promoter entry ,PTM:Phosphorylated on one or more of Ser-75 , Ser-99 , Ser-118 and Ser-134 in response to survival stimuli , which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118 , a site within the BH3 motif , leading to the release of Bcl-X (L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation , Ser-118 the major site of protein kinase A (CAPK) phosphorylation. ,similarity:Belongs to the Bcl-2 family. ,subcellular location:Upon phosphorylation , locates to the cytoplasm. ,subunit:Forms heterodimers with the anti-apoptotic proteins , Bcl-X (L) , Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity) . The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins. ,tissue specificity:Expressed in a wide variety of tissues. ,

Mitochondrion outer membrane. Cytoplasm . Colocalizes with HIF3A in the cytoplasm (By similarity) . Upon phosphorylation , locates to the cytoplasm. .

>>EGFR tyrosine kinase inhibitor resistance ;
>>Endocrine resistance ;
>>Platinum drug resistance ;
>>ErbB signaling pathway ;
>>Ras signaling pathway ;
>>cGMP-PKG signaling pathway ;
>>cAMP signaling pathway ;
>>Chemokine signaling pathway ;
>>Autophagy - animal ;
>>PI3K-Akt signaling pathway ;
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