Phospho MLKL (S358) mouse Monoclonal Antibody(6F8)
- Catalog No.:YM3670
- Applications:IF;IHC
- Reactivity:Human
- Target:
- MLKL
- Fields:
- >>Necroptosis;>>TNF signaling pathway;>>Salmonella infection
- Gene Name:
- MLKL
- Protein Name:
- MLKL
- Human Gene Id:
- 197259
- Human Swiss Prot No:
- Q8NB16
- Mouse Swiss Prot No:
- Q9D2Y4
- Immunogen:
- Synthetic Peptide of phospho-MLKL (S358)
- Specificity:
- phospho-MLKL (S358) protein detects endogenous levels of MLKL
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Monoclonal, Mouse
- Dilution:
- IF 1:50-200 IHC 1:100-200
- Purification:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- MLKL
- Observed Band(KD):
- 54kD
- Background:
- This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],
- Function:
- domain:The protein kinase domain is predicted to be catalytically inactive.,similarity:Belongs to the protein kinase superfamily.,similarity:Contains 1 protein kinase domain.,
- Subcellular Location:
- Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity). .
- Expression:
- Chondrocyte,Leukocyte,Lymph node,
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.
- Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.